Table 1 Molecular cytogenetic techniques, their resolution and validity for single-cell analysis of interphase/metaphase chromosomes (for more details see text)
From: Human interphase chromosomes: a review of available molecular cytogenetic technologies
| Approach | Resolution | MA* | IA** | SCA^ | PVC^^ | Refs |
|---|---|---|---|---|---|---|
| Cytogenetic banding analysis ("golden standard") | 5-7 Mb | + | - | + | + | [1] |
| FISH/MFISH/SKY | ||||||
| FISH/MFISH/SKY with centromeric probes | >0.3-1 Mb | +/- | + | + | +/- | [20–26, 30, 32, 35, 41, 42, 44, 46] |
| FISH/MFISH/SKY with site-specific probes | ~0.1-2 Mb | +/- | +/- | +/- | +/- | [45, 47–50] |
| FISH/MFISH/SKY with whole-painting probes | >5-10 Mb | + | - | + | + | [2, 3, 5, 6, 13, 36, 39, 40] |
| MCB | ||||||
| Metaphase MCB | ~2-5 Mb | + | - | + | + | [2, 13, 43, 45] |
| ICS-MCB | ~2-5 Mb | - | + | + | + | [23, 24, 26, 28, 29, 31–35] |
| Fiber FISH | >2.3 (2-3) kb | na | na | + | + | [51, 52] |
| Single-cell CGH | ||||||
| Standard CGH | 2-5 Mb | na | na | + | - | [53] |
| Array CGH | 0.03-1 Mb | na | na | + | - | [14, 54] |