Fig. 4

Morphology of the T. castaneum germinal vesicles at different stages of oocyte growth, squashed preparations of the stage I oocyte and the GVs from later stage oocytes. DIC, DAPI staining. Chromatin is dispersed and active transcription occurs at the stages II-III, before karyosphere formation that begins at the stage IV; V — late previtellogenic stage, a karyosphere capsule is formed; VII — vitellogenic stage, the capsule is fully developed, reaching its maximal complexity. Bars represent 5 μm for I—II; 10 μm for II; 20 μm for III—VIII